INTERACT-Bio is a shortened term for ‘Integrated sub-national action for Biodiversity’: Supporting implementation of National Biodiversity Strategy and Action Plans (NBSAP) through mainstreaming of biodiversity objectives across City-Regions. The project focuses on the promotion and enablement of the mainstreaming of biodiversity management between national governments and city-regions. Historical decisions by Parties to the Convention on Biological Diversity (CBD) and the global biodiversity targets. Aichi Biodiversity Targets have motivated these efforts. Going forward, sub-national government biodiversity action will support the aspirations and targets set out in the post-2020 Global Biodiversity Framework and the Long Term Approach to Mainstreaming biodiversity.