An interactive dialogue between INTERACT-Bio project city-regions in Tanzania (Dodoma, Arusha, Dar es Salaam and Moshi), national government, academia and NGOs was held on 3 October 2018. The purpose of the dialogue was to bring together national, subnational and local actors to deliberate the importance and benefits of urban nature, facilitate vertical and horizontal integration of initiatives of government actors as well as of non-governmental actors, provide an opportunity for knowledge sharing and information exchange on the mainstreaming of biodiversity and ecosystem services; and build the capacity of government officials and other actors.